GRANT REFERENCE

The invention described herein was made in the course of work under two grants from the USDA: CSRS grant No. 616-15-173 and competitive grant No. 5901-0410-8-0011.

BACKGROUND AND PRIOR ART

The 10-, 12-, 14- and 20-fluororetinoic acids and the 10-, 12- and 12,20-difluororetinoic acids have been reported in the literature: Machleidt et al, Justus Liebigs Ann. Chem., 1964, 674: 1; ibid, 1964, 679: 20; ibid, 1965, 681: 21 and U.S. Pat. No. 3,281,440. These compounds were found to have hypocholesterolemic activity. The 10- and 14- fluororetinals were synthesized in 1978 (Asato et al., J. Am. Chem. Soc., 1978, 100: 5957) and were shown to form fluorinated rhodopsin analogs.

The biological activities of the methyl and ethyl esters of 10-, 12- and 14-fluororetinoic acids have been evaluated in a chemically induced mouse papilloma test, and antipapilloma activity greater than the parent non-fluorinated esters were found for ethyl 12-fluororetinoate (Pawson et al., J. Med. Chem., 1979, 22: 1059-1067). Pawson et al have also synthesized (4-methoxy-2, 3,6-trimethylphenyl) nonatetraenoic acids, esters and amides (analogs of retinoic acid) bearing fluorine or the trifluoromethyl group on the polyene side chain (J. Med. Chem., 1979, 22: 1059-1067; ibid, 1981, 24: 101-104), and reported studies of antipapilloma activity.

The use of retinoic acid clinically against dermatoses was reported in 1959, and since that time it has been used in the treatment of acne, psoriasis, ichthyosiform dermatoses, dyskeratoses and various other skin diseases. See The Therapeutic Use of Vitamin A Acid, Proceedings of the International Symposium, Films, Switzerland, Jan. 27-29, 1975; and Acta Dermato-Venereologica, 1975, 55 (Supplement 74), 11-185.

Dietary vitamin A is required for growth, epithelial cell differentiation, vision and reproduction. The mechanism of action of vitamin A in vision is rather well defined, and either retinol or retinal, which are biologically interconvertible, can support vision. Retinoic acid, a natural biological metabolite of retinol, possesses equal growth promoting activity as retinol, but is not active in supporting vision or reproduction. No reports are available on the synthesis of N-retinoyl amino acids.

SUMMARY OF INVENTION

This invention provides a class of novel retinoid compounds having vitamin A activity while at the same time providing markedly reduced liver storage. Therefore, even though the level of vitamin A activity is somewhat less than retinol (vitamin A) or retinoic acid (vitamin A analog), the finding that the storage of the fluoro-substituted compounds of this invention in the liver is much less than with retinol or retinoic acid provides an important advantage in the oral administration of vitamin A activity for therapeutic purposes.

The compounds of this invention are characterized by having from 1 to 2 fluorines bonded to the C-15 carbon of retinol or retinoic acid. For example, the hydroxyl group of C-15 acid group of retinoic acid may be replaced with fluorine. Similarly, the C-15 end group of retinol may be modified by replacing both one of the hydrogens and also the hydroxyl group with fluorine.

DETAILED DESCRIPTION

The 15-fluoro-retinoid compounds of this invention can be represented by the following molecular formula: ##STR1## wherein R.sub.1 group, representing a C-15 group, has from 1 to 2 fluorines bonded to the C-15 carbon. For example, in the preferred C-15 fluoro derivatives, R.sub.1 is represented by one of the following groups: ##STR2## wherein F,C,O, and H are respectively fluorine, carbon, oxygen, and hydrogen. For example, the compounds of this invention include retinoyl fluoride and 15, 15-difluoroaxerophthene.

A useful fluorinating agent for preparing the compounds of the present invention is a N-diethylaminosulfurtrifluoride (DAST). The DAST reagent can be prepared by the method of L. N. Markovski et al., 1973, Synthesis, p. 787, or obtained commercially from Aldrich Chemical Co. It has been found that retinoic acid reacts readily with the DAST reagent to produce retinoyl fluoride in high yield. DAST also reacts with retinal to produce 15,15-difluoroaxerophthene.

Retinoyl fluoride, unlike most acyl chlorides, is stable in aqueous solution for several days and is hydrolyzed to retinoic acid only on heating in the presence of alkali or acid. It is not appreciably hydrolyzed to retinoic acid even in the presence of bicarbonate at 35.degree.-40.degree. C.

Retinoyl fluoride can be used as a starting material for the preparation of a new series of compounds, viz. N-retinoyl derivatives of glycine or other .alpha.-amino acids. In general, retinoyl fluoride will react with compounds containing a free NH.sub.2 group. The C-15 carbon of the retinyl moiety is bonded to the amino nitrogen. The general structure is as follows: ##STR3## wherein R.sub.2 is the carboxylic acid portion of the 60-amino acid.

Retinoyl fluoride has 22-36% of the biological activity of all-trans retinyl acetate by rat growth bioassay. However neither retinoyl fluoride nor any of its metabolites were found to be stored in appreciable amounts in the liver or other tissues.

EXAMPLE I

Synthesis of Retinoyl Fluoride

Retinoyl fluoride was prepared as follows:

300 Mg of retinoic acid was dissolved in 20 ml of warm diethyl ether. The solution was cooled in ice and 161 mg of N-diethylaminosulfurtrifluoride (DAST) dissolved in 1.0 ml of diethylether was added dropwise. (The DAST was prepared by the method of Markovski et al., 1973, Synthesis, p. 787.) The reaction mixture was warmed to room temperature and the solvent removed in vacuo. The orange residue was dissolved in 2 ml of acetone and immediately applied to three (20.times.20 cm) plates of silica gel and subjected to thin layer chromatography (t.l.c.) using hexane/diethylether (7:3 v/v) as the solvent mixture.

Retinoyl fluoride, which is orange-yellow in color, separated as the main band (R.sub.f =0.68). It was eluted with diethylether, and the solvent removed to give 240 mg of oil. Upon dissolving the oil in the minimum volume of pentane, and storing the solution at -20.degree. C., orange-yellow crystals m.p. 65.degree.-67.degree. C. were obtained: .lambda..sub.max 378 nm (hexane), E.sub.1cm.sup.1 %=1650. IR (KBr) 1788 (C=0), 1070 (C-F) cm.sup.-1. M.S. (m/e) 302 (M.sup.+), 287 (M.sup.+ -15 (CH.sub.3)), 282 (M.sup.+ -20(HF)), 267 (M.sup.+ -35(OF)), 259, 255 (M.sup.+ -47(COF)). .sup.19 F NMR (CDCl.sub.3) 14.74 ppm downfield from CFCl.sub.3. Anal. Calcd. for C.sub.20 H.sub.27 FO: C, 79.46; H, 8.94; F, 6.25. Found: C,79.42; H, 9.08; F, 5.92. NMR (CDCl.sub.3), .delta.1.03 (6H), 1.46 (2H), 1.71 (3H), 2.02 (3H), 2.05 (2H), 2.39/2.40 (3H), 5.73 (1H), 6.12 (1H), 6.17 (1H), 6.29 (1H), 6.34 (1H), 7.11 (1H).

Several other isomers were apparently formed. One of these isomers believed to be the 13-cis retinoyl fluoride showed a R.sub.f on t.l.c. of 0.80 and a .lambda..sub.max of 373 nm (hexane). HPLC (Whatman Partisil 10 (Type M9) column; 5% ethylacetate in hexane as the eluant at a rate of 4 ml/min, 370 nm detector) showed the retention time of the all-trans isomer to be 10.5 min and that of the 13-cis isomer to be 7.5 min.

EXAMPLE II

Synthesis of 15,15-Difluoroaxerophthene

15,15-Difluoroaxerophthene was prepared as follows:

100 mg of retinal was dissolved in 2-3 ml of CH.sub.2 Cl.sub.2 in a test tube, and the solvent was removed by passing argon through the solution. The oil was then treated with 56 mg of DAST. The mixture was kept at 80.degree. C. for 30 min in an atmosphere of argon. The mixture was cooled, diluted with 5 ml of diethyl ether and then hydrated with crushed ice. The ether layer was washed with water several times and then dried over Na.sub.2 SO.sub.4. The solvent was removed in vacuo and the red oil was dissolved in 1 ml of acetone. T.L.C. of the solution on silica gel using 4:1 (v/v) hexane-diethyl ether resulted in separation of 15,15-difluoroaxerophthene (R.sub.f =0.80) as the major band. 15,15-Difluoroaxerophthene was eluted with diethyl ether and the solvent removed in vacuo to give 60 mg of the compound as an orange-yellow oil.

.lambda..sub.max 325 nm, .about.390 nm. Mass spectrum: 306 (M.sup.+), 286 (M.sup.+ -20 (HF)), 271 (M.sup.+ -20-15 (CH.sub.3)). .sup.19 F NMR (CDCl.sub.3): 35.13 (d, J=19 Hz) ppm upfield from CF.sub.3 COOH.

In the foregoing Examples, the principal products are believed to be in the form of the all-trans isomers, while the minor products referred to in certain of the Examples are believed to be the 13-cis isomers. However, for the purpose of this invention, both the all-trans, or the 13-cis isomers may be used, or mixtures of the isomers. With respect to other C-15 substituted derivatives, which retain vitamin-A activity, other derivatives disclosed in the literature may be used. See Newton, et al., Cancer Research, 1980, 40: 3414-3425.

EXAMPLE III

Preparation of N-Retinoyl Glycine

Glycine (15 mg) was suspended in water (2 ml) to which solid NaHCO.sub.3 (15 mg) was added. A solution of retinoyl fluoride was prepared by dissolving 30 mg of retinoyl fluoride in 1 ml diethyl ether; 4 ml of methanol was added to the solution and then the glycine solution. The mixture was stirred at room temperature for 1 hour. The solution was diluted with water and any excess of retinoyl fluoride was extracted with diethyl ether. The yellow aqueous solution was then acidified with acetic acid and the retinoids were extracted with diethyl ether. The yellow extract was washed with water, dried over anhyd. Na.sub.2 SO.sub.4 and then evaporated to dryness in a rotary evaporator. The residual oil was dissolved in acetone and subjected to thin layer chromatography (TLC) on silica gel plates with 2:1 hexane-acetone. Two bands separated, a very small band (R.sub.f =0.71) that contained retinoic acid and a major broad band (R.sub.f =0.47) that contained N-retinoyl glycine. The compounds were eluted with CH.sub.2 Cl.sub.2 and ether. N-Retinoyl glycine was crystallized from CH.sub.2 CL.sub.2 pentane (1:10) at -20.degree. C. UV .lambda..sub.max 345 nm (methanol) which did not change in basic solution. In contrast, the .lambda..sub.max of retinoic acid in methanol shifted from 350 nm to 335 nm in presence of base. In the mass spectrum of N-retinoyl glycine, the following ions were prominent: 357 (M.sup.+), 339 (M.sup.+ -18(HOH), 255 (M.sup.+ -102, CONHCH.sub.2 COOH).

Following the above procedure and using 1:1 mole of retinoyl fluoride and the appropriate amino acid, the following were prepared:

N-Retinoyl lysine and N-retinoyl tryptophan.

DETERMINATION OF PROPERTIES

1. Determination of Biological Activity

Retinoyl fluoride was tested for vitamin A activity, as compared with retinyl acetate (vitamin A acetate).

The procedure described by Embree et al., Methods in Biochemical Analysis, 1957, p. 43-98 (Ed. D. Glick, Interscience, New York) and recommended by the U.S. Pharmacopeia XIII and XIV was followed.

Rats were made vitamin A-deficient and divided into 5 groups: one group served as control and received no supplement; another two groups received 5 and 10 .mu.g/day/rat of retinoyl fluoride and the remaining two groups received 2 and 4 .mu.g/day/rat of all-trans retinyl acetate. Rats in the control group died before the bioassay was over. The rats in the other four groups resumed growth. The gain in weight of the rats are shown in Table 1. The biological activity of retinoyl fluoride, as compared with retinyl acetate was found to be 22%.

                  TABLE 1
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    Biological Activity of Retinoyl Fluoride
    as Compared with Retinyl Acetate
                                            Bio-
                  Com-     Amount   Weight  logical
    Expt. No. of  pound    Dosed    Gain    Activity
    No.   Rats    Dosed    (.mu.g/rat/day)
                                    (g/wk/rat)
                                            (%)
    ______________________________________
     6        Retinyl  2          18.8    100
              Acetate
    6                  4          27.5
    7         Retinoyl 5          15.1     22
              Fluoride
    7                  10         19.8
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                  TABLE 2
    ______________________________________
    Comparison of Liver Storage of Vitamin A
    by Rats Receiving Retinyl Acetate and Retinoyl Fluoride
    Amount        Liver Vitamin A
            Received  Retinoic       Retinyl
                                            Retinoyl
    Compound
            in 35 days
                      Acid     Retinol
                                     Esters Fluoride
    Dosed   (.mu.g/rat)
                      (.mu.g/liver)
    ______________________________________
    Retinyl  140 .mu.g
                      Not      1.7 .mu.g
                                     40.3 .mu.g
                                            Absent
    Acetate           Detected
    Retinoyl
            5350 .mu.g
                      Not            Not
    Fluoride          Detected       Detected
    ______________________________________
     *Retinoic acid is difficult to detect even after massive dose (1 mg or
     more) of retinoic acid due to its rapid metabolism and excretion. Hence i
     is necessary to use labelled retinoid to detect retinoic acid. In order t
     study if retinoic acid was the sole metabolite of retinoyl fluoride, the
     following experiment was carried out.

                  TABLE 3
    ______________________________________
    Recovery of Radioactivity from the Livers
    of Rats Dosed with (11-.sup.3 H) Retinoyl Fluoride
           Total
           Radioactivity
                       Percent Distribution
    Time   Recovered   of Recovered Radioactivity
    ______________________________________
    1 hour 0.10%       Retinoic acid: 18.6%
                       Retinol: Not Detected
                       Retinyl Esters: Not detected
                       Retinoyl Fluoride: Not detected
                       Others (not characterized
                       81.4%
    3 hours
           0.42%       Retinoic acid: 49.6%
                       Retinol: Not detected
                       Retinyl esters: Not detected
                       Retinoyl Fluoride: Not detected
                       Others: 50.4%
    7 days 0.27%       Retinoic acid: 25.6%
                       Retinol: Not detected
                       Retinyl esters: Not detected
                       Retinoyl fluoride: Not detected
                       Others: 74.4%
    ______________________________________